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Calcium deposition Calcium deposition in the extracellular matrix was measured after 35 days of differentiation and exposure using a Calcium Assay Kit (Abcam, Cambridge, UK) according to the manufacturers instructions as described previously 19

Toxicology 339:7386 Ansari SM, Lama N, Blanc N, Bosilkovska M, Donelli A, Picavet P, Baker G, Haziza C, Ldicke F (2018) Evaluation of biological and functional changes in healthy smokers switching to the tobacco heating system 2.2 versus continued tobacco smoking: protocol for a randomized, controlled, multicenter study

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